Abo-Al-Ela, H. G., El-Magd, M. A., El-Nahas, A. F., and Mansour, A. A
الملخص العربي
Insulin-like growth factor 2 (IGF2) plays an important
role in muscle growth and it might be used as a marker for
the growth traits selection strategies in farm animals. The
objectives of this study were to detect polymorphisms in exon
10 of IGF2 and to determine associations between these polymorphisms
and growth traits in Egyptian water buffalo. PCRsingle-
strand conformation polymorphism (SSCP) and DNA
sequencing methods were used to detect any prospective polymorphism.
A novel single nucleotide polymorphism (SNP),
C287A, was detected. It was a non-synonymous mutation
and led to replacement of glutamine (Q) amino acid (aa) by
histidine (H) aa. Three different SSCP patterns were observed:
AA, AC, and CC, with frequencies of 0.540, 0.325, and 0.135,
respectively. Association analyses revealed that the AA individuals
had a higher average daily gain (ADG) than other
individuals (CC and AC) from birth to 9 months of age. We
conclude that the AA genotype in C287A SNP in the exon 10
of the IGF2 gene is associated with the ADG during the age
from birth to 9 months and could be used as a potential genetic
marker for selection of growth traits in Egyptian buffalo.
محمد رزق ابو المجد الغنام | Mohammed Rizk Abu El-Magd El-Ghannam | 8888