This study was conducted in Mycotoxin laboratory/College of Agriculture /University of Baghdad to evaluate seeds contamination of local and imported wheat with Deoxynivalenol(DON),and to test the ability of sodium sulfate and Fylax for detoxification of DON toxin . Results of fungal isolation showed that several fungi such as Alternaria. Aspergillus .Penicillium and Fusarium were associated with wheat samples for both local and imported wheat.Thin layer chromatography showed that 54.16%of Fusarium graminearum isolates were able to produce DON toxin on rice media .Results also showed that Fylax and Sodium sulfate inhibited the growth of the fungus F.graminearum and reduce toxin level from 45.6mg/g to0.27and 1.14 respectivily.