Mohammed F. AL Marjani ; Nebras R. Mohammed ;Suhad Y. Abd and Roaa F. Mansour
الملخص الانجليزي
Abstract
Manuscript History:
Received: 15 September 2015 Final Accepted: 22 October 2015 Published Online: November 2015
Key words:
Pseudomonas aeruginosa ,efflux pumps , MexAB-OprM ,MexXY.
*Corresponding Author
Mohammed F. AL Marjani
One Hundred and Twenty isolates of Pseudomonas aeruginosa were collected from different hospitals in Baghdad . The susceptibility of isolates was tested against colistin , results revealed that 27 isolates(22.5%) were resistant to colistin. The susceptibility of colistin resistant P. aeruginosa isolates to different antibiotics was evaluated by disk diffusion method. Results showed that all isolates were resistant to Amoxicillin and carbenicillin. The resistance to Gentamycin and Lomefloxacin were 70.3% and 59.2% respectively. Norfloxacin , Levofloxacin and Ciprofloxacin were found to be the most effective agents against Colistin resistant P. aeruginosa isolates. Sixity isolates (27 Colistin resistant isolates and 33 Colistin sensitive isolates) were choosen for detect efflux pumps by using Ethidium Bromide – Agar Cartwheel Method (EtBr-CW) , the result showed that 46 isolates (%76.6) give positive result .Polymerase Chain Reaction (PCR) technique was used to detect the mexA , oprM and mexY gene . The results showed that oprM found in 50% of Colistin resistant isolates , mexA found in 56.25% of isolates and mexY found in 88.9% of isolates . Detection of gene expression was performed done by using qRT-PCR technique after RNA extraction from colistin resistant isolates and cDNA synthesis . The result showed that gene expression were high expression in efflux pumps especially in MexXY efflux pump
نبراس رضا محمد | Nebras Rada Mohammed | 19335