عنوان المقالة: Inhibition gene expression MexAB-OprM and MexXY efflux pumps of Pseudomonas aeruginosa(XDR) by Novel inhibitors Levofloxacin, Silver nanoparticles and Beta rays.
Abstract
This study was achieved in order to determine the inhibition of gene expression of MexAB-OprM and MexXY efflux pumps by using different Novel inhibitors including: Levofloxacin, AgNPs and Beta rays emitted by Tl208 and Sr90 on P.aeruginosa (XDR). Efflux pumps are responsible for pumps various kinds of antibiotics including quinolones(fluoroquinolones),Aminoglycosides, BLactam and Polymyxin in Pseudomonas aeruginosa (XDR). Levofloxacin utilized as Novel inhibitor to MexXY and MexAB-OprM effiux pumps to P.aeruginosa(XDR),the percentage of killing determined.Detection of gene expression by using qRT-PCR,improved no gene expression of MexXY and MexAB-OprM when adding Levofloxacin 50 mg/ml when study gene expression of mexX and mexB encoded to MexXY and MexAB-OprM efflux pumps. Silver nanoparticles(AgNPs) used as New inhibitor of MexXY and MexAB-OprM effiux pumps of P.aeruginosa(XDR), the percentage of killing calculated , the survey of gene expression by utilizing qRT-PCR was determined. The results exhibit no gene expression of the MexXY and MexAB-OprM when adding Silver nanoparticles 100 mg/ml with study of mexX , mexB and compared with control (without treating). Beta rays utilized as new inhibitors emitted of Tl208 radiosource with activity 1 𝛍ci, dose 12.93*10-10 KGy for 3 hr.; also exposure to Sr90 radiosource with activity 1 𝛍ci, dose 1.937*10-10 KGy for 3 hr. and exposure to Sr90 radiosource with activity 9 𝛍ci, dose 6.3*10-10 KGy for 3 hr. The results exposition Beta radiation P.aeruginosa(XDR) showed the number of colonies less than the control with rise the percentage of killing of P.aeruginosa(XDR), also it found the morphology of the colonies changed compared with the original(control). Detection gene expression of P.aerginosa(XDR) prior exposition to Levofloxacin, AgNPs, Beta rays and after exposition to Levofloxacin, AgNPs, Beta rays was achieved by qRT-PCR technique, RNA extraction of P.aerginosa(XDR),synthesis of cDNA ,calculate gene expression according to Livak equation to detect gene expression of MexXY and MexAB-OprM efflux pumps with study of mexB and mexX gene. The results exhibit gene expression prior exposition to Levofloxacin, AgNPs and Beta rays were highly in MexXY and MexAB-OprM efflux pumps of P.aerginosa(XDR) but fewer after exposition to Levofloxacin, AgNPs and Beta rays.The Levofloxacin, AgNPs, Beta rradiation were ecompetence to killing P.aeruginosa and qualification
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as novel inhibitors to inhibit gene expression of MexXY and MexAB-OprM efflux pumps P.aerginosa(XDR).
نبراس رضا محمد | Nebras Rada Mohammed | 19335