يهدف هذا الكتاب الى دراسة أنتشار وطرق الكشف الجزيئية لبكتریا الايروموناس المعزولة من مختبر الصحة العامة في مدینة الحلة، العراق. حیث اظهرت النتائج انه من من مجموع ٨٢٢ عینه خروج مأخوذة من مصابين بالاسهال ، أن ١٣ عینة فقط كانت عائدة لبكتریا الايروموناس. باستخدام بادئ خاص ببكتریا تم تأكید التشخیص باستخدام تفاعل البلمرة المتعدد. أظهرت الدراسة قابلية الأنواع المختلفة من هذه البكتريا كانت متعددة المقاومة للمضادات الحياتية وخاصة مضاد التتراسايكلين.
الملخص الانجليزي
The aim of this work was to investigate the prevalence of tetracycline resistant Aeromonas hydrophila isolated from clinical sources in Hilla city, Iraq. Clinical Samples were collected from fecal specimens from patients who suffering from diarrhea.. Results revealed that out of 822 fecal samples, 13 isolates (1.58%) were belonged to Aeromonas spp. However, other bacterial isolates belonged to other genera similar to Aeromonaswere also recovered. Out of 13 Aeromonas spp., eight A. hydrophila isolates (61.53%) were obtained, while the other isolates were distributed as: four isolates of A. salmoncidia (30.76%), and one of A. sobria. Isolation and detection of A. salmoncidia species was first recorded in Iraq. The susceptibly of A. hydrophila (NO. 8) to several antibiotics was determined using disk diffusion test (DDT). Results showed that 5 isolates (62.5%) were Multi-drug resistant (MDR) and three isolates (S2, S3, and S5,) were sensitive to most of antibiotic classes tested. The MIC of A. hydrophila to tetracycline was also detected using ager dilution method according to CLSI guidelines. Results found that only 2 isolates (25 %) were resistant to tetracycline. The MIC ofthese isolates ranged from 0.25-16 μg/ml. This result confirms tetracycline resistance by these isolates when tested using DDT.
أ.د. علاء هاني الجراخ | Prof. Alaa H. Al-Charrakh | 19949