عنوان المقالة:Uterine Implantations and Litter Size Alteration in Anti-inhibin and eCG-hCG Treated Virgin Pregnant Female Rats
حاتم عبدالكريم جبر الشويلي | Hatim Alshwilly | 4913
نوع النشر
مجلة علمية
المؤلفون بالعربي
Online International Interdisciplinary Research Journal
الملخص العربي
To investigate the comparative roles of anti-inhibin and eCG-hCG treatment on uterine implantation sites and litter size in virgin female rats, 120 virgin cycling female rats, aged 56 days and weighted 150-170 g., were randomly assigned to three equal groups. At late metaestrus and 54h later, the females were supplemented with intraperitoneal injection of 1>g anti-inhibin and 100>l normal saline, respectively (AI group), 20IU eCG and 10IU hCG, respectively (eCG group), and 100>l twice, respectively (Control). After mating, blood samples were obtained daily from 6 females of each group, at the first 4 days, for hormonal assay (FSH, LH, 17Bestradiol, and prolactin). At day 5 of pregnancy, 6 females from each group, were injected with 1% (w/v) pontamine blue (500 >l, iv). After 15 min., the females were sacrificed for counting of uterine implantations. The remaining 18 females continued to the end of gestation. Litter numbers were counted. Serum FSH in AI females registered higher levels than others throughout the four days of pregnancy, whereas LH levels showed no differences, except day one which recorded significant elevation in eCG group. Prolactin levels increased significantly in AI group throughout the four days of pregnancy. Estradiol concentrations showed insignificant differences among groups except day one which showed significant elevation in AI group. Uterine implantation sites increased significantly in AI group compared with others, also, eCG group recorded higher number than control. In conclusion treatment of virgin female rats with anti-inhibin, at late metastrus, increases the reproductive efficiency and multiple births.
تاريخ النشر
27/01/2016
الناشر
Online International Interdisciplinary Research Journal
رابط الملف
تحميل (209 مرات التحميل)
رابط خارجي
www.oiirj.org
الكلمات المفتاحية
Anti-inhibin, eCG, fertility, Implantation sites, Litter size
رجوع