The present study examined the antioxidant activity of methanolic extracts of propolis samples from different regions of Algeria. Total phenol content (TPC) was determined by using Folin-Ciocalteau Reagent. Total flavonoid content (TFC) was determined by using aluminum chloride method. Resulting ranged between (210.884±0.754-19.626±0.301 mg/100g) and (262,338±0.810-81.141±0.538 mg/100g) crude extract of propolis, respectively. Thereafter, evaluate propolis’ antioxidant activity, measured by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and Total antioxidant activity was measured, based on the phosphomolybdenum assay (PM). A novel system to evaluate the Algerian propolis’ antioxidant activity is reported. The methods use a cyclic and square wave voltammetry. These techniques are realized to compare the results from spectroscopic method and to electrochemically characterize the propolis polyphenols. Our results justify the use of as a natural source of antioxidant compounds could be used in the prevention of free radical-related diseases.