عنوان المقالة:Stability of Anticancer Drug 5-Fluorouracil in Aqueous Solution: An Assessment of Kinetic Behavior Stability of Anticancer Drug 5-Fluorouracil in Aqueous Solution: An Assessment of Kinetic Behavior
رزاق عبدالزهرة إبراهيم حمود العامري | Razzaq Abd Al-Zahra Ibrahim Alamery | 11283
نوع النشر
مجلة علمية
المؤلفون بالعربي
د.رزاق عبدالزهرة إبراهيم،د.فلاح شريف عبد سهيل ،د.حسين كاظم الحكيم
المؤلفون بالإنجليزي
Razzaq Abd Al-Zahra Ibrahim, Falah Shareef Abed Suhail, Hussein Kadhem Al-Hakeim
الملخص العربي
Spectroscopic measurements of pharmaceutical compound 5-fluorouracil (5-FU) drug was achieved in solvents with different polarity. The drug gave clear absorption peak at 259, 269, 270 and 266 nm in hexane, dimethyl sulfoxide (DMSO), N, N-dimethylformamide (DMF) and ethanol, as well as phosphate buffer respectively, which was consistent with the standard values in literatures. The results of extension coefficient (ε) and wave length (λmax) showed reduction in polar medium as compared to non-polar medium. The calibration curve of 5-FU drug was achieved by using serials solutions dis- solved in phosphate buffer (pH = 7.4 and T = 37 °C) within the range of 1×10– 6 ~ 1×10– 4 M. The sta- bility of 5-FU drug was studied in phosphate buffer at pH = 5, 6, 7, 7.4 and 8 with 1×10– 5 M and T = 37 °C, according to the equation of first-order reaction. The hydrolysis of 5-FU disappeared at alkaline solu- tion, but had noticeable hydrolysis in acidic solutions with the rate constant 25, 14 and 20 at pH of 5, 6 and 7 respectively. The calculation of molar extension coefficient and half-life (t1/2) showed same sequence of 5-FU hydrolysis. Then, rearrangement of obtained results offered complicated reversible equilibrium state by the combination between thermodynamic and kinetic behaviors of 5-FU hydro- lysis; with Keq = 8.46, 6.11 and 142.8 at pH of 5, 6 and 7 respectively. The acidic hydrolysis of 5-FU occurred spontaneously within free energy (ΔG) did not exceed 10 kJ/mol, which meant the electro motive forces of interactions were weak, notable to release energy such as Van der Waals forces or hydrogen bonding
الملخص الانجليزي
Spectroscopic measurements of pharmaceutical compound 5-fluorouracil (5-FU) drug was achieved in solvents with different polarity. The drug gave clear absorption peak at 259, 269, 270 and 266 nm in hexane, dimethyl sulfoxide (DMSO), N, N-dimethylformamide (DMF) and ethanol, as well as phosphate buffer respectively, which was consistent with the standard values in literatures. The results of extension coefficient (ε) and wave length (λmax) showed reduction in polar medium as compared to non-polar medium. The calibration curve of 5-FU drug was achieved by using serials solutions dis- solved in phosphate buffer (pH = 7.4 and T = 37 °C) within the range of 1×10– 6 ~ 1×10– 4 M. The sta- bility of 5-FU drug was studied in phosphate buffer at pH = 5, 6, 7, 7.4 and 8 with 1×10– 5 M and T = 37 °C, according to the equation of first-order reaction. The hydrolysis of 5-FU disappeared at alkaline solu- tion, but had noticeable hydrolysis in acidic solutions with the rate constant 25, 14 and 20 at pH of 5, 6 and 7 respectively. The calculation of molar extension coefficient and half-life (t1/2) showed same sequence of 5-FU hydrolysis. Then, rearrangement of obtained results offered complicated reversible equilibrium state by the combination between thermodynamic and kinetic behaviors of 5-FU hydro- lysis; with Keq = 8.46, 6.11 and 142.8 at pH of 5, 6 and 7 respectively. The acidic hydrolysis of 5-FU occurred spontaneously within free energy (ΔG) did not exceed 10 kJ/mol, which meant the electro motive forces of interactions were weak, notable to release energy such as Van der Waals forces or hydrogen bonding
تاريخ النشر
10/09/2018
الناشر
Nano Biomed. Eng.
رقم المجلد
10
رقم العدد
3
رابط DOI
DOI: 10.5101/nbe.v10i3.p224-234
الصفحات
224-234
رابط الملف
تحميل (235 مرات التحميل)
رابط خارجي
http://www.nanobe.org
الكلمات المفتاحية
Stability of 5-FU; Physicochemical properties; Beer-Lambert relationship; Chemical affinity
رجوع