عنوان المقالة:التاثير الوقائي للزيت الخام للحبة السوداء في الغدة الكظرية لذكور الفئران البيض المعاملة بجرعة سامة واطئة من الباراسيتامول Protective Effect of Crude Oil of Nigella sativa on Adrenal gland in Male Albino Mice Treated with Low Toxic Dose of Paracetamol
Abstract: Paracetamol is one of the most common nonprescription drugs in the world, it can be used by pregnant women as it is relatively safe. However, overdose may have significant side effects on the functions and tissues of different body organs. On the other hand, the black seeds have beneficial effects in various central nervous system disorders including epilepsy, neurotoxicity and memory impairment. Nigella sativa oil can be consumed by patients with peptic ulcer as it protected gastric mucosa from the ulcerative effect of alcohol. Also, the aqueous extract of Nigella sativa seeds has a protective effect against damage of pancreatic beta cells and the ethanolic extract of Nigella sativa helps alleviate stress and increases anoxia tolerance in mice. Therefore, the aim of current study was to investigate the possible protective effects of crude oil of black seeds against low toxic dose paracetamol-induced histomorphometry alterations in the adrenal glands of male mice. Methods: The animals were divided into one control group and three experimental groups, the former were given 0.3 ml from 0.9% of NaCl only, whereas the mice in experimental groups were given single sub-lethal dose of paracetamol ( 300 mg/kg body weight ) and Nigella sativa oil ( 0.3 ml ) alone and together. Results: the study showed a significant (P<0.05) reduction in the adrenal gland weight of the fourth group recorded and wasn’t significant for the third group. In addition, the results revealed a significant increase (P<0.05) in average thickness of cortex and its three zones while the diameter of medulla significantly decreased (P<0.05) in the second group, in the third group. The administrated animals in the crude oil of Nigella sativa significantly decreased (P<0.05) in the diameter of the adrenal gland and thickness of zona fasciculata and average diameter of medulla as compared with control group. Therefore, the administration of low toxic dose of paracetamol induced stress- related changes in the adrenal gland. However, these changes can be antagonized by oral administration of crude oil of Nigella sativa seed
وسن نجم عبد السادة الربيعي | Wasan Najim Abdul-Sada Al-Rubeai | 1023