عنوان المقالة:دراسة تاثير مستخلص البن اليمني ضد سمية الاوكراتوكسين A Protective effect of Yemeni green coffee powder against the oxidative stress induced by Ochratoxin A
Qais A. Nogaima,1, Lakshmi Sai Pratyusha Bugatab,1, Prabhakar PVb, Utkarsh A. Reddyb, Mangala Gowri Pb, Indu Kumari Sb, Mahboob Mb,*
الملخص الانجليزي
The current study focusses on knowing the antioxidant effects of green Yemeni coffee powder on reducing the
oxidative stress that was induced by Ochratoxin A in kidney, liver and brain of rats. The grouping of female
albino Wistar rats was into 5 groups (5 rats/group). Rats of Group 1 designated Vehicle Control (only water),
Group2 (10 mg/kg Ochratoxin A); Group 3 designated Low dose (2000 mg/kg Coffee+10 mg/kg Ochratoxin A);
Group 4 designated High dose (4000 mg/kg Coffee+10 mg/kg Ochratoxin A); Group 5 designated Coffee
Control (1000 mg/kg Coffee) and orally administered with the above test materials repeatedly every day for 28
days. On termination of the study, liver, brain and kidney tissues were collected after dissection, oxidative stress
biomarkers (Levels of Lipid Peroxidation and Reduced Glutathione, activities Superoxide Dismutase, Catalase
and Glutathione Reductase enzymes) and histopathological studies were carried out. Treatment of Ochratoxin A
alone (group 2 rats) significantly increased malondialdehyde content, catalase, and glutathione reductase activities
with a decrease in the activity of superoxide dismutase enzyme and reduced glutathione level and in
brain, kidney and liver. Whereas, low dose coffee (group 3) and high dose coffee (group 4) rats showed dosedependent
increase in antioxidant and less histopathological alterations. Concomitant treatment of Yemeni green
coffee powder and Ochratoxin A brought dose-dependent protective effects against oxidative stress which was
induced using Ochratoxin A in liver, brain, and kidney tissues of female rats.
أ.د./ قيس عبدالله عبدالله نجيم | Qais Abdullah Nogaim | 795