Mohammed F. AL- Marjani, Haidar J. AL- Kafaji, Haider Y. AL-Murayati
الملخص العربي
During a 4 months period a dominated pattern of multiple resistance to
antibiotic of clinical E. coli isolates which isolated from Central Medicine City
hospital / Baghdad ,were analyzed and compared with the multiple resistance
pattern of sweges isolates .the results revealed that the multiple resistance
pattern of clinical isolates was : CX - CRO – CE- AMC - TR and the pattern
of sweages was : CX – CRO- CE- AMC.
The isolates which resistant to Cefotaxime , Ceftazidime and Imipenem were
selected to detect the ability to Metallo ß -lactamases production (MßLs) .Ten
clinical isolates and five swege isolates were able to produce MßLs ,and these
isolates were tested to blaIMP-1 by using polymerase chain reaction( PCR) and
using Primer :
F 5- ACC GCA GCA GAG TCT TTG CC – 3
R 5-ACA ACC AGT TTT GCC TTA CC _ 3
Two isolate appeared to carry blaIMP-1 gene after electrophoresis of the
results on 1.2% agarose.
The results of Bacterial Conjugation experiment between Clinical and Swege
isolates showed that the Metallo β-Lactamases were unable to transferred in this
process.
حيدر يوسف احمد المراياتي | Hider yousif Ahmed AL-Murayati | 1066