عنوان المقالة:Effects of Dietary Selenium Supplementation on Seminiferous Tubules and SelW, GPx4, LHCGR, and ACE Expression in Chicken Testis Effects of Dietary Selenium Supplementation on Seminiferous Tubules and SelW, GPx4, LHCGR, and ACE Expression in Chicken Testis
Abstract We investigated the effects of dietary selenium (Se)
supplementation on the development of chicken testis and the
expression of selenoprotein W (SelW), glutathione peroxidase4
(GPx4), luteinizing hormone/choriogonadotropin receptor
(LHCGR), and angiotensin converting enzyme
(ACE). Sixty roosters were assigned randomly into the control
group fed with a basic diet (containing 0.3 mg Se/kg) and the
experimental group fed with a diet (containing 0.6 mg Se/kg).
The testes were collected individually at age of 6, 9, and
12 weeks. Se was supplemented in chicken feed for 15 days
before sampling. The results indicated that dietary Se affected
the number of cells in the seminiferous tubules and viability of
Sertoli cells in vitro culture. SelWand GPx4 expression in the
testes increased significantly in the experimental group compared
to that in the control group. LHCGR expression in the
testes increased significantly in the experimental group after
12 weeks compared to that in the control group. In contrast,
ACE expression was inhibited in the experimental group compared
to that in the control group. These results suggest that
dietary supplementation with Se improved development of the
seminiferous tubules at the cellular level and that SelW, GPx4,
LHCGR, and ACE are involved.
الملخص الانجليزي
Abstract We investigated the effects of dietary selenium (Se)
supplementation on the development of chicken testis and the
expression of selenoprotein W (SelW), glutathione peroxidase4
(GPx4), luteinizing hormone/choriogonadotropin receptor
(LHCGR), and angiotensin converting enzyme
(ACE). Sixty roosters were assigned randomly into the control
group fed with a basic diet (containing 0.3 mg Se/kg) and the
experimental group fed with a diet (containing 0.6 mg Se/kg).
The testes were collected individually at age of 6, 9, and
12 weeks. Se was supplemented in chicken feed for 15 days
before sampling. The results indicated that dietary Se affected
the number of cells in the seminiferous tubules and viability of
Sertoli cells in vitro culture. SelWand GPx4 expression in the
testes increased significantly in the experimental group compared
to that in the control group. LHCGR expression in the
testes increased significantly in the experimental group after
12 weeks compared to that in the control group. In contrast,
ACE expression was inhibited in the experimental group compared
to that in the control group. These results suggest that
dietary supplementation with Se improved development of the
seminiferous tubules at the cellular level and that SelW, GPx4,
LHCGR, and ACE are involved.
تاريخ النشر
22/02/2016
الناشر
Biological Trace Element Research - Springer Science+Business Media New York 2016
أ.م.د. احمد خالد احمد | Dr. Ahmed Khalid Ahmed AL-ANI | 1289