عنوان المقالة: Overproduction of fibrinolytic enzyme from mutant Staphylococcus aureus by Chemical mutagenesis
نبراس رضا محمد | Nebras Rada Mohammed | 7995
نوع النشر
مقال علمي
المؤلفون بالعربي
المؤلفون بالإنجليزي
Nebras Rada Mohammed 1) and Salwa Jaber AL-Auadi
الملخص الانجليزي
Abstract The study design of cases are Cross-sectional study that descriptive study by internal comparsion of 500 isolates collected from different provenance of human infections including 280(56%) from tonsils , 100(20%) nose, 40(8%) tumors, 17(3.4%) urine, 27(5.4%) skin and 36(7.2%) blood, the isolation and identification achieved by using Vitek2-GP and Genotypic detection PCR to confirmitive identification of S.aureus isolated from tumors. Fibrinolytic enzyme (Staphylokinase) assay done on plasma agar plate and Casein agar plate to determine fibrinolysis, caseinolysis on medium , the result exhibit 260 (56%) isolates possess production of staphylokinase, inclusive throat 100 (38%), Blood 36(15%), Tumors 38(10%), Skin 27(23%) and Nose 59(23%) production staphylokinase; 180 (69%) isolates of Vancomycin resistance S.aureus (VRSA) and Methicillin resistance S.aureus (MRSA) produces Staphylokinase . The chemical mutagenesis was achieved via exposition VRSA S.aureus and MRSA S.aureus to different doses of chemical mutagen including Hydroxylamine(HA) in (10,20,30) mg ; Acridine Orange (AO) in (30,40,50,60) mg and Ethylemethansulfonate (EMS) in (0.01, 0.02, 0.03, 0.04, 0.001, 0.002)ml, results of mutagenesis on viability with increase production of staphylokinase were of Hydroxylamine in 10mg with viability 85, as well Acridine Orange (AO) give rise to increase production of Staphylokinase in 30 mg with viability 75 and Ethyle methan sulfonate (EMS) in 0.001ml, 0002 ml with viability 73. Study chemical mutagenesis as well one after exposition S.aureus (VRSA) and S.aureus (MRSA) to mutagen turn into sensitive to Vancomycin renowned Vancomycin Sensitive S.aureus (VSSA) and sensitive to Methicillin renowned 2 Methicilline Sensitive S.aureus (MSSA) howevere, continue possess higher productivity of Staphylokinase (fibrinolytic enzyme).
تاريخ النشر
04/01/2020
الناشر
نبراس رضا محمد
رقم المجلد
رقم العدد
رابط الملف
تحميل (489 مرات التحميل)
الكلمات المفتاحية
Key words: Mutage, Mutagen, Genotoxicity, Thrombolytic enzyme, Acridine Orange, Ethylemethansulfunate, Hydroxyleamine
رجوع