عنوان المقالة: The Effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on Leishmania tropica promastigotes
نبراس رضا محمد | Nebras Rada Mohammed | 7995
نوع النشر
مقال علمي
المؤلفون بالعربي
المؤلفون بالإنجليزي
Nebras Rada Mohammed1, Hanna Salih Sabaa 2, Khawla Hori Zghair 3, Raad Said Abd
الملخص الانجليزي
Abstract This work evaluated the effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on Leishmania tropica promastigotes in vitro. The experiment included a control and tetra-plicate of L. tropica promastigotes exposed to Nd:YAG laser in 500 pulse between each pulse 6 sec, in wavelength 1060 A°; the effect of Semiconductor laser in (5, 10, 20, 30) minute , with wavelength 532 nm; also use He-Ne laser in (5, 10 , 20 , 30 ) minute , with wavelength 6328 A°; and effect of Beta and Gamma irradiation by 137Cs isotopes , in two doses (1.105607×10-7 Gy , for 2hr, and in dose 1.650992×10-7 Gy for 3hr), cesium isotopes that give two type of decay Gamma and Beta Rays. The effect of Nd:YAG, Semiconductor, He-Ne laser and Beta, Gamma irradiation on the viability of L. tropica promastigote was determined using the colorimetric MTT assay, the number of viable cells of L. tropica was fewer than control (without exposure to laser and irradiation ). Nd:YAG laser, Semiconductor laser, He-Ne laser and Beta , Gamma irradiation was efficient in killing L. tropica promastigotes in vitro, and the remain of L. tropica after exposure to laser and irradiation was devoid flagellum and may affect the capability of infection.
تاريخ النشر
03/01/2017
رقم المجلد
رقم العدد
رابط الملف
تحميل (489 مرات التحميل)
الكلمات المفتاحية
Keywords: Leishmania attenuation, cutaneous leishmaniasis, laser types, laser wavelengths.
رجوع