عنوان المقالة:Ahmed OM, Ashour MB, Fahim HI, AbouZid SF, Ahmed RG, Mohamed A.A.G., 2016. Ameliorative effects of Punica Granatum juice and fruit extracts against 7,12-dimethylbenz (A) anthracene and carbon tetrachloride-induced cardiorenal toxicity in albino rats. SM J Biol. 2(2), 1011.
Ahmed OM, Ashour MB, Fahim HI, AbouZid SF, Ahmed RG, Mohamed A.A.G.,
الملخص العربي
Background: Both 7,12-dimethylbenz (a) anthracene and carbon tetrachloride are xenobiotics that are
implicated in various toxicological effects including carcinogenesis. Punica granatum constituents have antioxidant
and anti-inflammatory activities. This study is conducted to assess the preventive effects of pomegranate (Punica
granatum) juice, seeds extract, husk extract and their mixture on 7,12-Dimethylbenz (A) Anthracene (DMBA) and
Carbon Tetrachloride (CCl4)-induced perturbed kidney and heart function and integrity.
Methods: Fifty male albino rats received single oral dose of DMBA (50 mg/kg b.w.) followed by consecutive
oral doses of CCl4 (3 mg/kg b.w. /week) beginning from the third week for thirteen weeks and ten animals kept
as normal control. Pomegranate (Punica granatum) juice (10 ml/kg b.w.), seeds extract (400 mg/kg b.w. /day),
husk extract (400 mg/kg b.w. /day) and their mixture (10 ml/kg b.w. /day) were orally administered to DMBA/CCl4-
treated rats beginning from the 1st day of the experiment.
Results: The obtained data revealed a significant elevation in serum urea, uric acid, creatinine and K levels
and decrease in Na concentration in DMBA/CCl4-administered animals. In addition, there are an elevation of
lipid peroxidation (MDA) and Nitric Oxide (NO) in kidney tissue homogenate and depletion in antioxidant namely
GSH, GPx, GST, SOD and Total Antioxidant Capacity (TAC). Furthermore, the activity of serum enzymes related
to heart functions as CK, CK-MB, LDH and AST were increased in DMBA/CCl4-administered animals. These
data reflect a cardiorenal toxicity which was confirmed by histopathological perturbations in the kidney and
heart tissues. These deleterious changes of DMBA/CCl4 were significantly improved by treatment with Punica
granatum juice and fruit extracts.
Conclusion: Based on the obtained result, it can be concluded that pomegranate has antioxidant properties
and synergistic effects of its bioactive compounds in juice and extract mixture may prevent DMBA/CCl4-induced
heart and kidney impaired function and integrity.
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