ABSTRACT
Twenty one local isolates of Aspergillus niger were screened for their ability to produce α-amylase using solidstate
fermentation (SSF), A. niger N11 local isolate exhibited maximum efficiency for amylase production, this
isolate had been chosen for mutation by CO60 Gamma ray. Several food wastes were used, such as wheat bran,
potato peel, banana peel and corn cob. The highest yield of amylase produced from A. niger N11 mutant isolate
was obtained with wheat bran when used as a substrate. Soluble starch and Ammonium nitrate (1%w/w) used
as the best additional carbon and nitrogen supplements respectively. The optimum pH, temperature and
incubation period for α-amylase production by mutant isolate were found to be 5.5, 30°C and 6 days,
respectively.
تاريخ النشر
11/02/2014
الناشر
Current Research in Microbiology and Biotechnology
علي جبار رشك | Ali Jabbar Rashak | 1599