عنوان المقالة:التقدير الطيفي لدواء الثايمين هيدروكلوريد بواسطة ازوتتة مع البروكايين امايد The Spectrophotometric Determination of Thiamine Hydrochloride Drug by Coupling with Diazotized Procainamide
For the determination of thiamine hydrochloride (THC) in both pure and formulated forms, a simple,
sensitive and accurate spectrophotometric method was described. The technique is based on diazotization
of the primary amine group of Procainamide with sodium nitrite and hydrochloric acid followed by
combining with thiamine hydrochloride to create Light red azo dye showing peak absorption at (502) nm.
Beer's law has followed the concentration range (2.5-40) μg.mL‾¹,with (0.1490) μg.mL‾¹ detection limit.
The molar absorptivity and Sand ellʾs sensitivity were detected to be respectively (7285.0320)
L.mol‾¹.cm‾¹and (0.0463) μg.cm‾². The method for determining in thiamine hydrochloride pharmaceutical
preparation has been applied successfully.
Keywords: Thiamine hydrochloride, Spectrophotometric determination, Procainamide, Diazotization
مها عبدالستارمحمد نجيب | Maha abd alsattar mohammed najeeb | 2443