The aim of this study is to prove the antifungal and antibacterial activity of Ginko Bioloba GKB from glycerin
nanocapsules and nanoemulsions. GKB was formulated as spray formulation for topical application using ethyl alcohol
95%/glycerol/water based formulations. GKB spray was evaluated for their particle size distribution, pH, content
uniformity, content per spray, in vitro release and antifungal activity. The antifungal activity of was determined by Agar
well-diffusion method. The formulae were tested for their skin permeation and the stability of the formulations. The
results showed uniform formulae with nano-sized particles, pH compatible with the topical formulation and higher drug
content. Diffusion studies of the optimized formulations through the abdominal rabbit skin showed higher penetrated
drug over a period of 24 h. Stability studies indicated that formulations were stable. Skin irritation studies confirmed the
compatibility of spray formulae with skin. Agar well-diffusion method showed that GKB was effective compared with
fluconazole. The inhibition zones for all formulae were significant as compared with standard fluconazole. The results
obtained showed that the topical spray formulation could be a promising and innovative therapeutic system as topical
antifungal for the transdermal administration of GKB. The developed formulation can be used for delivery from spray
formulation. This study proved the local activity of GKB as antifungal drugs.
احمد عبداللطيف | Ahmed Abdellatif | 1187