Ahmed A. H. Abdellatif , Shaaban K. Osman , Mohd Tajudin Mohd Ali , Fouad H. Darras
الملخص العربي
It is beneficial to PEGylate the Octreotide (OCT), in order to
enhance its biological and physiochemical properties, and to give a
longer circulation time. OCT was covalently linked to methoxy-
polyethylene-glycol with amine terminal (Me-PEG-NH 2 ). First, Me-
PEG-NH 2 was succinylated with succinic anhydride, then the reaction
was established at low pH to react the Me-PEG-COOH selectively to
the N-terminus of OCT. The covalent attachment of OCT to Me-PEG-
COOH was confirmed by the increase in molecular weight by
MALDI-ToF mass spectroscopy, which yielded the expected
molecular weight of Me-PEG-OCT. The integrity of Me-PEG-OCT
and its purity were examined using HPLC. The percent conversion to
Mono-PEG-OCT was 71%, as determined by 1 H-NMR. The formed
PEGylated-OCT may serve as a linker for coating different
nanoparticles which forms a hydrophilic protective layer around the
nanoparticles, which prevent and block the opsonization process
around the nanoparticles.
تاريخ النشر
20/04/2016
الناشر
Pharmaceutical Journal of Innovative Drug Research and Development
احمد عبداللطيف | Ahmed Abdellatif | 1188