A sensitive, selective, and validated spectrofluorimetric method is being described
for the determination of penicillin G. The method utilizes this drug (as a source of the
amine moiety) to react with acetylacetone and formaldehyde through the Hantzsch
reaction. The faint yellow product of dihydropyridine derivatives was measured at an
emission wavelength of 481 nm (after excitation at 416 nm). Linear calibration graphs
were obtained in the range of 0.2-200 µg/mL. The experimental limits of detection and
quantitation were 0.170 and 0.515 µ/mL, respectively. The relative standard deviation
(n=5) at the 100 µg/mL level were 6.11 for intra-day precision and 9.42 for inter-day
precision. The method was successfully applied in the analysis of pharmaceutical
formulation; the recovery was quantitative, and the result obtained agreed with those
obtained by another reported method.
احمد عبداللطيف | Ahmed Abdellatif | 1187