عنوان المقالة:Study of the Cytotoxic Effects of Aflatoxin on Hematopoietic Stem Cells Study of the Cytotoxic Effects of Aflatoxin on Hematopoietic Stem Cells
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 8260
- نوع النشر
- أخرى
- المؤلفون بالعربي
- المؤلفون بالإنجليزي
- Musleh Mohammed Hamada1*, Al-Kubaisi Salah M.A.2 and Al-Ouqaili Mushtak T.S.3
- الملخص الانجليزي
- Aflatoxins (AFs) are secondary metabolites having a high cytotoxic potential and have an active carcinogenic compound. This study aimed to isolate hematopoietic stem cells (Precursors of mononuclear stem cells) from human umbilical cord blood. Collection of umbilical cord blood samples from cord blood was accomplished immediately after delivery, while the placenta is intrauterine by used closed system method. The mononuclear cells (MNCs) were obtained from umbilical cord blood by using Buffy coat method. The previous cells have been Hematopoietic Stem Cells (HSCs) used in the cytotoxicity test of AF extract. The mononuclear cells were cultured in DMEM medium with 10% FCS. An experimental study was conducted in vitro on these cells to confirm the toxic effects of AF with different concentrations of AFs. The results showed that the inhibition rate increases seriously with the increase of AF concentration. Aspergillus flavus in peanut produces the highest concentration of AF, while in soil, contamination with crude oil A. flavus produces the least concentration of AF. Additionally, the concentration of 1 μg /ml of AF extracted from the aflatoxigenic isolates which can kill 100% of hematopoietic stem cells.
- تاريخ النشر
- 25/03/2019
- الناشر
- Research Journal of Biotechnology
- رقم المجلد
- 14
- رقم العدد
- رابط الملف
- تحميل (151 مرات التحميل)
- رابط خارجي
- https://www.worldresearchersassociations.com/BiotechSpecialIssueMarch2019/9.pdf
- الكلمات المفتاحية
- Aflatoxins, Cytotoxicity, Lymphocyte, Stem cells, Umbilical cord blood.