عنوان المقالة:Isolation and identification of potential probiotic Lactobacillus species from feces of infants in southwest Iran Isolation and identification of potential probiotic Lactobacillus species from feces of infants in southwest Iran
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 8261
- نوع النشر
- مجلة علمية
- المؤلفون بالعربي
- المؤلفون بالإنجليزي
- Nabi Jomehzadeh , Hazhir Javaherizadeh , Mansour Amin , Morteza Saki , Mushtak T.S. Al-Ouqaili
- الملخص الانجليزي
- Objectives To evaluate the potential probiotic properties of Lactobacillus strains isolated from feces of infants and also to determine their antimicrobial activity against some enteropathogenic bacteria. Methods The Fecal samples were prepared from 120 infants aged less than 24 months. In total, 105 Lactobacillus strains were identified by phenotypic tests. Thirty isolates were randomly selected to study their potential probiotic properties. These isolates were examined for resistance to acid (pH: 2.5, 2 h) and bile (oxgall 0.3%, 8 h), adhesion to HT-29 cells, antibiotic susceptibility, and antimicrobial activities. Results On basis of 16S rRNA sequencing, 30 isolates identified as Lactobacillus fermentum (n = 11; 36.7%), Lactobacillus plantarum (n = 9; 30%), Lactobacillus rhamnosus (n = 6; 20%), and Lactobacillus paracasei (n = 4; 13.3%). All tested strains survived at acid and bile conditions. Six Lactobacillus strains revealed high adherence to HT-29 cells. Three strains including the L. fermentum (N2, N7), and the L. plantarum (N20) showed good probiotic potential and inhibited the growth of Yersinia enterocolitica ATCC 23715, Shigella flexneri ATCC 12022, Salmonella enterica ATCC 9270, and enteropathogenic Escherichia coli (EPEC) ATCC 43887. The antibiotic resistance test showed that all the isolates were susceptible to tetracycline, and chloramphenicol. Conclusions Lactobacillus strains like L. fermentum (N2, N7), and the L. plantarum (N20), could be potential probiotic, but further in vitro and in vivo studies on these probiotic strains are still required.
- تاريخ النشر
- 19/05/2020
- الناشر
- International Journal of Infectious Diseases
- رقم المجلد
- 96
- رقم العدد
- ISSN/ISBN
- 1201-9712
- رابط DOI
- https://doi.org/10.1016/j.ijid.2020.05.034
- رابط الملف
- تحميل (151 مرات التحميل)
- رابط خارجي
- https://www.sciencedirect.com/science/article/pii/S1201971220303416#!
- الكلمات المفتاحية
- Lactobacillus , Adhesion , Probiotic , Enteropathogens