عنوان المقالة: MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION
جبار سلمان حسان | Jabbar Salman Hassan | 774
- نوع النشر
- مقال علمي
- المؤلفون بالعربي
- المؤلفون بالإنجليزي
- Thanaa R. Abdulrahman , Dhefaf R. Mahdi , Ayad M. Gaidan & Jabbar S. Hassan
- الملخص الانجليزي
- Background: Methicillin resistance Staphylococcus aureus (MRSA) infection related to community has been enhanced through the world. One of the significant cytotoxins elaborated by a few strains of S. aureus is the Panton Valentine leukocidin (PVL), coded by two genes, lukS- PV and lukF-PV which is element of toxin that created pores in the membranes of cells, the role of PVL in the disease process severity of Staphylococcus aureus till known is debated. Amis: This study was planned to detect the Panton Valentine leucocidin (PVL)genes and MRSA isolated from Burn wound infection in community and hospital acquired settings. Material and methods: Cross-sectional study involved 200 patients who presented with burn wounds from third-degree or fourth-degree. The bacteria from all samples were undergone to Cefoxitin disc diffusion testing utilization a 30 μg cefoxitin disc for methicillin resistant determination. Following DNA extraction, conventional polymerase chain reaction (PCR) was used to detect PVL genes. Results: Twenty-eight samples were positive culture for Staph. aureus, of which 19 (67.8%) patients presented with CAI, and 9 (32.1%) belong Hospital acquired infection A total of 23 (82.1%) showed methicillin resistance. Out of 28 isolates, 7(25%) isolates were harboring PVL gene, all of which were Methicillin resistance community acquired infection. None of S. aureus isolates from hospital acquired infection had PVL. Conclusion: This study indicated high prevalence of PVL among community acquired infection MRSA isolates, and the lack of this gene in Hospital acquired infection.
- تاريخ النشر
- 09/12/2020
- الناشر
- EJMCM, International House
- رقم المجلد
- 7
- رقم العدد
- 9
- ISSN/ISBN
- 2515-8260
- الصفحات
- 254-259
- رابط الملف
- تحميل (0 مرات التحميل)
- الكلمات المفتاحية
- Keywords: Panton Valentine Leukocidin, Methicillin resistance, community acquired infection, Hospital acquired infection.