عنوان المقالة: The Role of DNA Gyrase (gyrA) in Ciprofloxacin- Resistant Locally Isolates Pseudomonas aeruginosa in Al-Khadhmiya Teaching Hospital Baghdad, Iraq
Jabbar S. Hassan, Montaha A. Al-Safar and Thanaa Rasheed Abdul Rhman
الملخص الانجليزي
Ciprofloxacin comprise a medically effective and generally utilized class of broad-spectrum antibiotics;
as a result of the intensive use of this type of therapy, bacteria have become resistant, lead to minimize
the usage of such bactericides in field of management as well as treatment of microbial illness.
Pseudomonas aeruginosa one of important pathogens that case wide spectrum of infections, the
evolution of ciprofloxacin-resistant Pseudomonas aeruginosa were notify. In this investigation 50 clinical
isolates of Pseudomonas aeruginosa were collected, antibiotic susceptibility test for ciprofloxacin by
using the Kirby–Bauer technique as well as MIC was performed to all these isolates. Specific primers
sequences for detection of Topoisomerase II (gyrA) genes were used by conventional PCR then, PCR
product were treated with Sac II enzyme at 37°C for 2 h, then restriction bands were recovered by gel
electrophoresis. From Fifty Pseudomonas aeruginosa isolates enrolled in this study, 33(66%) show
sensitive to Ciprofloxacin, 17(34%) reported resistant to Ciprofloxacin. RFLP-PCR showed that out
of 17 isolates resistant to Ciprofloxacin mutation inDNA gyrase (GyrA) were detected in six (35.2 %)
isolated while the reminder 11 (64.7%) has no mutation. The presence of mutations in Topoisomerases
II genes mediate Ciprofloxacin defeat as therapy for Pseudomonas aeruginosa. The current venture,
were made to demonstrate Topoisomerase II genes (GyrA mutations in Pseudomonas aeruginosa that
could be responsible for molecular mechanism of Ciprofloxacin resistance.
جبار سلمان حسان | Jabbar Salman Hassan | 774