Walaa M. Elakbawy, Sanaa M. M. Shanab, Emad A. Shalaby, Rehab M. Hafez
الملخص الانجليزي
The present study aimed to evaluate the genetic divergence of canola and tomato in vitro plantlets cultured on MS medium
supplemented with the plant growth regulators produced from some algae cultivated on BG110 for Cyanobacteria species
(Anabaena oryzae and Nostoc muscorum) and BG11 for Chlorophyta species (Chlorella vulgaris) (as control media), grown
on treated sewage wastewater concentration (TSW combined with control media) (100, 75, and 50%) and diferent concentrations of nitrogen (0, 0.5, 2, and 4 of NaNO3 in BG11) for Chlorophyta. The experiment was conducted in triplicate, and
cultures were incubated at 25±1 °C under continuous aeration (1.25 L/min), 16:8 h light and dark cycle and illumination (40
µE/m2
/s), and harvested at the end of the exponential phase. The HPLC was used for growth regulator (auxins and cytokinins)
analysis in diferent algal treatments. Apical buds of canola and tomato seedlings were dissected and transferred to MS media
with algal extract containing the optimum concentration of benzyl adenine (BA) and indole acetic acid (IAA) and diferent
concentrations of synthetic hormones.Basal solidifed MS medium and/or dimethyl sulfoxide (DMSO) were considered as
control. Genetic stability among the produced in vitro plantlets of both plant species was evaluated by inter-simple sequence
repeat (ISSR) and random amplifed polymorphic DNA (RAPD) markers. The whole banding pattern of in vitro plantlets of
both species revealed their low divergence compared to controls when cultured on medium with algal-based plant hormones.
This work indicated that the use of sewage wastewater as media for cultivation of the investigated algae is safe, suitable,
cheap, and not expensive methods when compared with the ordinary used media.
عماد احمد شلبي | Emad Ahmed Shalaby | 901