Aim and objective: The discovery and development of new natural antiviral compounds which exhibit various antiviral activities
are required. The aim of this investigation is to assess the potential use of the red seaweed Asparagopsis taxiformis as a new source
of anti H5N1 agent.
Methods: The seaweed was collected from Marsa Matrouh, Mediterranean Sea, Egypt during spring season, the effects of
successive extracts and the pure compounds from the investigated alga on H5N1 virus were performed using plaque reduction
assay. In addition, the mechanism of action of promising extract on the virus adsorption and replication was determined.
Chromatographic and spectroscopic analyses were used for the identification of chemical structure of active compound(s) isolated
from the studied seaweed.
Results: The obtained results showed that petroleum ether and water algal extracts exhibited high antiviral activity (>99.9%) and
the mode of action of extracts was not correlated with virus replication but with its adsorption process.The isolated pure compound
was identified as 6-methyl-Δ
22
-stigmasterol-2, 3 di acetate and its antiviral activity (for H5N1)was tested. Pure compound showed
antiviral activity reached 56% at 100 µg/ml.
Conclusion: The obtained results suggests that crude extracts and isolated active compound from A. taxiformis has the capacity to
protect people against pandemic H5N1preventing virus adsorption to the human host cells. Recommendation for testing the extracts
and pure compounds from the studied seaweed as potential inhibitor of COVID-19.
تاريخ النشر
01/12/2020
رقم المجلد
رقم العدد
رابط DOI
: https://doi.org/10.22270/ujpr.v6i2.573
الكلمات المفتاحية
Antiviral activity, Asparagopsis taxiformis, H5N1 virus, mode of action.
عماد احمد شلبي | Emad Ahmed Shalaby | 1407