عنوان المقالة:Novel strain MN907806 S.aureus expressed high production of Thrombolytic enzyme(Staphylokinase) Novel strain MN907806 S.aureus expressed high production of Thrombolytic enzyme(Staphylokinase)
Study design of this study is Descriptive / Cross-sectional study designs for 500 isolates, Analytic study design is Case-Control study design for (1) case is Novel strain MN907806. Study seeting in Baghdad hospital at 2019/2020 including 280 (56%) Tonsils, 100(20%) Nose, 40(8%) Tumors, 17(3.2%) Urine, 27(5.4%) Skin (Acne) and 36(7.2%) Blood, identification by using Viteck2-GP and genotypic detection of conventional PCR for 16srRNA (ribosomal units 30s) reveals all isolates are S.aureus. Quantitative screening of Thrombolytic enzyme (staphylokinase) of Novel strain MN907806 S.aureus done on plasma agar plate in order to determine fibrinolysis zone around well, results give positive result for over production of thrombolytic enzyme.
Genotypic detection of sak gene achieved to Novel strain MN907806 S.aureus with denaturation 94°C(1min), Annealing 52 °C (1min), Extension 72°C(1min) and Final extension 72°C for (10 min) by PCR (Polymerase Chain Reaction) in order to determine possess Novel strain MN907806 S.aureus for sak gene and send PCR product to NICEM/USA ABI3730XL Applied Biosystems to study DNA Sequencing of sak gene, the results analyzed according to NCBI (National Center for Biotechnology Information) data and Alignments of amino acid sequence were analyzed with the same software, the results give novel strain have mutation transversion in locus 156 from Guanine(G) into Adenine (A) that leading to convert amino acid in locus 148 from Glycine (G) to Serine (Ser) leading to increase production of thrombolytic enzyme compared with Control (wild type strain). Protein drawing by Mega 6 program software program for two forms Alpha helix and Beta sheets that explain found change in protein(thrombolytic enzyme), record strain in NCBI (Gene Bank in America USA) with name Nebras R. Mohammed.
Abstract
Study design of this study is Descriptive / Cross-sectional study designs for 500 isolates, Analytic study design is Case-Control study design for (1) case is Novel strain MN907806. Study seeting in Baghdad hospital at 2019/2020 including 280 (56%) Tonsils, 100(20%) Nose, 40(8%) Tumors, 17(3.2%) Urine, 27(5.4%) Skin (Acne) and 36(7.2%) Blood, identification by using Viteck2-GP and genotypic detection of conventional PCR for 16srRNA (ribosomal units 30s) reveals all isolates are S.aureus. Quantitative screening of Thrombolytic enzyme (staphylokinase) of Novel strain MN907806 S.aureus done on plasma agar plate in order to determine fibrinolysis zone around well, results give positive result for over production of thrombolytic enzyme.
Genotypic detection of sak gene achieved to Novel strain MN907806 S.aureus with denaturation 94°C(1min), Annealing 52 °C (1min), Extension 72°C(1min) and Final extension 72°C for (10 min) by PCR (Polymerase Chain Reaction) in order to determine possess Novel strain MN907806 S.aureus for sak gene and send PCR product to NICEM/USA ABI3730XL Applied Biosystems to study DNA Sequencing of sak gene, the results analyzed according to NCBI (National Center for Biotechnology Information) data and Alignments of amino acid sequence were analyzed with the same software, the results give novel strain have mutation transversion in locus 156 from Guanine(G) into Adenine (A) that leading to convert amino acid in locus 148 from Glycine (G) to Serine (Ser) leading to increase production of thrombolytic enzyme compared with Control (wild type strain). Protein drawing by Mega 6 program software program for two forms Alpha helix and Beta sheets that explain found change in protein(thrombolytic enzyme), record strain in NCBI (Gene Bank in America USA) with name Nebras R. Mohammed.
نبراس رضا محمد | Nebras Rada Mohammed | 18967