عنوان المقالة: Isolation, purification, and characterization of lectins from medicinal plant Combretum glutinosum seeds endowed with analgesic and antiulcer properties
Eva H. Naser a , Ahmed H. Idries a , Sara A.A. Elmubarak a , Maha B. Dafalla a , Yusria E. Abdelrahim a , Entsar A. Abdalrhman a , Bashir M. Ahmed b , Makarim Elfadil M. Osman a , Amna K.E. Awadallah a , Reem M.A. Ebrahim a , Ashraf O. Abdellatif c , Haseeba A. Saad d , Emadeldin H.E. Konozy
Abstract
, Cape Coast, Ghana
article info
Article history:
Received 19 April 2024
Received in revised form
19 June 2024
Accepted 2 August 2024
Available online xxx
Handling Editor: Dr B Friguet
Keywords:
Lectin
Combretum glutinosum
Purification
Characterization
Analgesic
Antiulcer
abstract
In the pursuit of safer and more effective treatments, there is a growing interest in plant-derived
compounds, particularly lectins, because of their diverse pharmacological properties. This study
focused on isolating, purifying, and characterizing lectin from Combretum glutinosum seeds (CGSLs) to
assess its potential as an analgesic and antiulcer agent. CGSL extraction involved defatting and buffer
extraction, followed by purification using ammonium sulfate fractionation and fetuin-agarose affinity
column chromatography. The isolectins (iso-CGSLs), each consisting of 60 kDa and 57 kDa heterodimeric
subunits, displayed glycoprotein properties with a 40 % neutral sugar content. They exhibited peak activity at 55 C and remained stable for up to the fifth day at room temperature. The activity exhibited a
pH dependence, peaking between 7.5 and 10.5, and all seemingly operated independently of metal ions.
CGSL, at optimal doses ranging from 6 to 12 mg/kg, had significant analgesic effects on acetic acidinduced writhing and hot plate tests in mice. Evaluation using 0.7 % acetic acid resulted in notable
pain reduction across all doses (P < 0.05). The analgesic effect of lectin was partially reversed by naloxone
(a morphine antagonist), indicating partial involvement of the opioid receptor system. Furthermore,
CGSL exhibited antiulcer effects in ethanol-induced gastric ulcer models in rats, highlighting its therapeutic potential as a natural alternative for analgesic and antiulcer treatments
يسرية الشيخ عبدالرحيم عبدالواحد | Yusria Elshiekh Abdalrahim Abdalwahed | 323