In the work, fifty soil samples were cultivated on selective Gauze agar medium plates containing carboymethyl cellulose as the sole carbon source. Thirty-two samples showed similarity to the morphological characteristics of Streptomyces bacterium which were tested for cellulolytic activity using the 3,5-Dinitrosalicylic acid reagent. Four efficient Streptomyces isolates were selected for their high cellulolytic activity which was recorded after 12 hours of incubation in the early logarithmic phase of growth. The four isolates attained their optimum cellulose activity after 96 hours of incubation with an enzyme activity of (0.55 + 0.1, 0.53+ 0.12 and 0.49 + 0.22 U/mL) by the Streptomyces isolates AA-17-1, AA-17-19 and AA-17-22 respectively with recording a specific enzymatic activity of (0.68 + 0.23 U/ml) of the Streptomyces isolate AA-17-32. The result showed, a supreme activity of the Streptomyces purpureus AA-17-32 to reduce (20 g) of paper waste to about (0.78 U/ml). After applying further morphological and biochemical analysis it's recognized that, the Streptomyces isolate AA-17-32 belonged to the species Streptomyces purpureus. It concludes that this method for the production of cellulose can be used for the sustainable development of sugar production from the degradable of waste materials.
Abstract
In the work, fifty soil samples were cultivated on selective Gauze agar medium plates containing carboymethyl cellulose as the sole carbon source. Thirty-two samples showed similarity to the morphological characteristics of Streptomyces bacterium which were tested for cellulolytic activity using the 3,5-Dinitrosalicylic acid reagent. Four efficient Streptomyces isolates were selected for their high cellulolytic activity which was recorded after 12 hours of incubation in the early logarithmic phase of growth. The four isolates attained their optimum cellulose activity after 96 hours of incubation with an enzyme activity of (0.55 + 0.1, 0.53+ 0.12 and 0.49 + 0.22 U/mL) by the Streptomyces isolates AA-17-1, AA-17-19 and AA-17-22 respectively with recording a specific enzymatic activity of (0.68 + 0.23 U/ml) of the Streptomyces isolate AA-17-32. The result showed, a supreme activity of the Streptomyces purpureus AA-17-32 to reduce (20 g) of paper waste to about (0.78 U/ml). After applying further morphological and biochemical analysis it's recognized that, the Streptomyces isolate AA-17-32 belonged to the species Streptomyces purpureus. It concludes that this method for the production of cellulose can be used for the sustainable development of sugar production from the degradable of waste materials.
الدكتور محمد الجرادين | Mohammad Aljaradin | 11447