عنوان المقالة:عزل مشتقات الفانيلين من ثمار شجرة البرقوق باستخدام تقنيات الاحيائبة والكيميائية Biotechnology and Biochemical Analysis Techniques to isolate Vanillin derivatives for Plum Fruit Tree (I
علاء جعفر محراث / ايمان اسماعيل يوسف /طارق حسين مغير
English Authors
Alaa J.Mahrath / Eman I. Yousif /Tariq H.Mgheer
Abstract
This application studied some biochemical and technological techniques to identify
,isolate and purified some of biological molecules from fruits of Plum tree . The study
involved drying the sample with freeze dryer for 24 hrs . Then samples were performed with
Soxhlet apparatus by protic solvent (PSI) , aprotic solvent (ASI) ,and aprotic–protic solvent
interaction( PASI) were used to extracted the molecules . Then recovered the excess solvent vy
evaporation and condensation. The sample directly transformed to the flash column
chromatography technique (FCC)and monitored by Thin layer chromatography (TLC) to
isolate m-methoy-p-hydroxy benzoic acid (vanillic acid) or (VA) and m-methoxy-p-hydroxy
ethyl Benzoate (Ethyl Vanillate) or EV in about 95% pure state. These molecules AV and
EV elucidated by spectroscopic tools ( UV-Vis , ATR-IR ,1H-NMR, 13C-NMR , and Mass
spectrometry) .
علاء جعفر محراث | Alaa J.Mahrath | 5689