Ahmed Mahdi Saeed, Abbas Shebeeb Al-kadumi and Noor Jassem Mohammed Ali
Abstract
تم تقدير فيتامين C طيفيا بتكوين معقداته مع الذهب
Abstract
Determination of vitamin C (Ascorbic acid) via complex formation with gold using UV-VIS spectrophotometry and atomic absorption spectrophotometry (AAS) was investigated in this research. The absorption maxima of vitamin C – gold complex was found to be at (599 nm) and this wavelength was selected for the analysis of vitamin C as standard and in formulated samples. The optimum conditions were investigated through the study of different parameter such as concentration, time, pH, temperature, and complex formula. Complex stability constants was found (2.6192 x106). The methods are linear in the range of (2-45 μg/mL) and (1–22 μg/mL) with R2 values of (0.9993, 0.9992) for UV-VIS and AAS methods respectively. The relative standard deviation (0.48, 0.82), detection limits (0.24, 0.09) and recoveries (100.34 and 100.32 %) for the UV-VIS and FAAS respectively. The methods were applied for the estimation of the active gradient of the vitamin C in different samples of formulated dosage. Accuracy of methods was validated by mean percentage recovery which was found to be in the acceptable range.
Publication Date
8/1/2017
Publisher
Research Journal of Pharmaceutical, Biological and Chemical Sciences
احمد مهدي سعيد محمد | Ahmed Mahdi Saeed Mohammed | 8530