عنوان المقالة:Anticancer Drug 5-Fluorouracil in Aqueous Solution by Differential Pulse Polarography: An Assessment of Optimum Conditions Anticancer Drug 5-Fluorouracil in Aqueous Solution by Differential Pulse Polarography: An Assessment of Optimum Conditions
The potassium phosphate buffer as a supporting electrolyte of anticancer drug (5-fluorouracil (5-
FU)) was the best among solutions of sodium phosphate buffer and Britton Robinson buffer in
differential pulse polarography (DPP) at pH = 7.0 and T = 37 °C. The changes of temperature did
not effect on inactivity of the supporting electrolyte (potassium phosphate buffer at T = 10-50 °C),
and pH of the solution did not exceed 2% of each 5 °C (at pH = 7.0, by modified thermostat cell).
However, the frequency measurements showed clear effect of temperature on diffusion current (IP /
μA) of the chemotherapy compound in the range of 20-50 °C and under primary conditions. Then,
the polarography measurements of 5-FU drug (at 10 μM, pH = 7 and T = 37 °C) gradually led to
the optimum conditions: deposition potential =–0.9 V; drop size = 9.0 mm3
; deposition time = 15.0
s; equilibration time = 5.0 s; pulse amplitude = 100 mV; pulse time = 7.0 ms; voltage step = 6 mV;
voltage step time = 0.3 s; and sweep rate = 20.0 mV/s. The thermal assessment of 5-FU drug (after
achievement of the optimum conditions) in a new thermostat vessel at HMDE by DPP showed that
the reaction of 5-FU molecules represented pseudo first order reaction, instead of first order); the
secondary waves of 5-FU drug may be due to formation of molecular complexes in aqueous solution
and the reduction of 5-FU molecules at mercury surface electrode appeared as physisorption, instead
of chemisorption.
Abstract
The potassium phosphate buffer as a supporting electrolyte of anticancer drug (5-fluorouracil (5-
FU)) was the best among solutions of sodium phosphate buffer and Britton Robinson buffer in
differential pulse polarography (DPP) at pH = 7.0 and T = 37 °C. The changes of temperature did
not effect on inactivity of the supporting electrolyte (potassium phosphate buffer at T = 10-50 °C),
and pH of the solution did not exceed 2% of each 5 °C (at pH = 7.0, by modified thermostat cell).
However, the frequency measurements showed clear effect of temperature on diffusion current (IP /
μA) of the chemotherapy compound in the range of 20-50 °C and under primary conditions. Then,
the polarography measurements of 5-FU drug (at 10 μM, pH = 7 and T = 37 °C) gradually led to
the optimum conditions: deposition potential =–0.9 V; drop size = 9.0 mm3
; deposition time = 15.0
s; equilibration time = 5.0 s; pulse amplitude = 100 mV; pulse time = 7.0 ms; voltage step = 6 mV;
voltage step time = 0.3 s; and sweep rate = 20.0 mV/s. The thermal assessment of 5-FU drug (after
achievement of the optimum conditions) in a new thermostat vessel at HMDE by DPP showed that
the reaction of 5-FU molecules represented pseudo first order reaction, instead of first order); the
secondary waves of 5-FU drug may be due to formation of molecular complexes in aqueous solution
and the reduction of 5-FU molecules at mercury surface electrode appeared as physisorption, instead
of chemisorption.
رزاق عبدالزهرة إبراهيم حمود العامري | Razzaq Abd Al-Zahra Ibrahim Alamery | 11052