Jassim Hamza K. Al-Masoudi, Mohammed A. K. Al-Saadi, Maysa Salih AL-Shukri
Abstract
This work aims at the genotyping characterization for Chlamydiapneumoniae in patients with cardiovascular diseases in Karbala Province-Iraq.Blood samples were collected from each subject. Bacterial DNA was extracted from patient fresh blood. Samples were subjected for molecular detection of C. pneumoniae by using convential polymerase chain reaction (PCR) depending on 16SrRNA. Also, virulence genes for C.pneumoniae including outer membrane protein (omp1) and heat shock protein (hsp) were detected byPCR technique. Five samples (7.75%) revealed positive results for C. pneumoniae by PCR technique in blood samples of patients group, whereas all control samples were negative. Regarding virulence genes, four samples (80%) out of five samples expressed positive for omp1, whereas only two samples (40%) expressed positive result for hsp.
Publication Date
4/3/2015
Publisher
International Journal of Research Studies in Biosciences
د. ميساء صالح مهدي الشكري | Dr. Maysa Salih Mahdi Al-Shukri | 10994