عنوان المقالة:فعاليه المستخلص القلوي لنبات Opuntia polyacantha المضاد لسرطان الثدي للإنسان Anti-Cancer Activity of Opuntia polyacantha Alkaloid Extract on Human Breast Cancer Cell Line
أ.م.د.لبنى عبد العظيم البياتي. أ.م.فرح طارق. م.م.هديل علاء م .ياسر حيدر
English Authors
Lubna Abdulazeem , Farah Tareq Al-Alaq , Hadeel Alaa Alrubaei , Yasir H. Al-Mawlah , Wasan K. Alwan
Abstract
تم استخلاص القلويدات من نبات الصبار والتي لها فعاليات بايولوجيه مضاده للسرطان وقد تمراستخلاصها باستخدام الايثانول ٨٠٪ والكلوروفورم ثم قياس فعالياتها بطريقه إلى MTTلقياس القابلية السميه للمستخلص وبالتراكيز تحت الدراسه.
Abstract
Cancer as one of the major killer disease is getting tremendous concern in research and demands a proactive strategy for protection and treatment. Research on plant secondary metabolites revealed a promising safe alternative anti-cancer agents. Alkaloids are naturally occurring organic nitrogen containing compounds that have many biological activities including anti-cancer. Alkaloids were extracted from Opuntia polyacantha plant by methanol (80%) and chloroform and then estimated quantitatively and qualitatively. The MTT viability assay used to determine alkaloids cytotoxicity by using MCF-7 and WRL-68 cell lines. The results showed that the MCF-7 cell line growth was significantly decreased by 52.7% at 400μg/ml total alkaloid concentration with 91.89% viability of WRL-68.
لبنى عبد العظيم مجيد موسى البياتي | Lubna Abdulazeem Al-Bayati | 4558