عنوان المقالة:التشخيص الجزيئي للفطر المسبب للذبول الفرتسليومي على الزيتون Molecular Diagnosis of Verticillium dahliae Kleb; the Causal Agent of Verticillium Wilt of Olive
Ali Kareem Mohammed Al-Taae* and Huda Hazim Wafi Al-Taae
Abstract
The present study was conducted on olive wilt disease caused by Verticillium dahliae Kleb, which has
recently distributed in Iraq and is considered as one of the most important and devastating diseases on
olive trees in Iraq. The results of the field survey, carried out in certain regions of Ninevah province during
the years 2004 and 2005, showed that the highest disease incidence and severity were found in Alsada
region in 2005 and reached 48.72% and 0.29; while the lowest values were recorded in Khorsybad and
reached 30.14% and 0.26, respectively.
For the first time in Iraq, the Polymerase Chain Reaction (PCR) was used to diagnose the different isolates of the
pathogen using two specific pairs of primers (FVD and RVD). The PCR results showed that V.dahliae Kleb is
the prevalent disease in Iraq which produced DNA fragment bands of 330 bp which was expected for these
primers. The samples were also processed for detection using (NESF18s and NESR28s) primers in Nested PCR
where these primers are used in common detection of all Verticillium spp. and the products were subjected to
another PCR reaction using specific primers for V.dahliae (FVD and RVD) .The results of these tests confirmed
that V.dahliae is the common species and it was detected in healthy trees (unclear symptoms), indicating that this
test is to be used to detect the disease in its early stages.
KEYWORDS: Olive ,Verticillium wilt,Polymerase Chain Reaction (PCR).
ا.د.علي كريم محمد الطائي | Prof.Dr.Ali Kareem Mohammed Al-Taae | 9834