عنوان المقالة:نظام للتحديد السريع وتقييم كثافة السابونين في الانسجة النباتية A SYSTEM FOR RAPID LOCALIZATION AND INTENSITY EVALUATION OF SAPONINS ACCUMULATION IN PLANT TISSUES
MELA G. ABDUL-HALEEM, Dr. LOAY E. GEORGE, Dr. ANSAM G. ABDUL-HALEEM
Abstract
A fluorescence microscopy considered as a powerful imaging tool in biology and medicine. In addition to
useful signal obtained from fluorescence microscopy, there are some defects in its images such as random
variation in brightness, noise that caused by photon detection and some background pixels in the acquired
fluorescence microscopic images appear wrongly auto-fluorescence property. All these practical limitations
have a negative impact on the correct vision and analysis of the fluorescent microscope users. Our research
enters the field of automation of image processing and image analysis using image processing techniques
and applying this processing and analysis on one of the very important experiments in biology science.
This research is devoted to develop a system based on digital image processing methodology to localize
and assess the concentration of saponins accumulation in plant tissues using Fluorescence microscopic
image. The proposed system involved preprocessing steps than to make the region of interest more obvious
and reflects the saponins accumulation area. Also, the system introduces a simple mathematical way for
concentration assessment, and it was justified through the test results. It includes building a system to get
microscopic images with best appearance and no defects. It determines the saponins accumulation sites in
leaves, rhizomes and shoot apex of Y. gloriosa Variegata and their in vitro cultured tissues (Calli, direct and
indirect regenerated shoots and rhizomes/roots). Statistical analysis is performed using a computer to get
the mean and median of saponins intensities in each part, and finally perform a comparison between them
to determine which part can record the highest intensity level of saponins. The results showed high ability
of the system to determine the locations and intensity of saponins in the different parts of the plant. It
performs the statistical analysis very quickly. In in vitro culture, it was found that callus treated with
Thidiazuron (TDZ) in a combination with benzyl aminopurine (BA) and naphthyl acetic acid (NAA) after 3
weeks of culture had the highest level of saponins accumulation, while the leaves of intact plant recorded
the second highest accumulation of saponins.
ميلا غازي عبد الحليم | Mela G. Abdul-Haleem | 4554