Rakan A. Hussein1, Thekra A.Hamad2 and Nehan Bahaaldden Jafar1
Abstract
The ability to produce enzymes such as hemolysins is an important virulence factor for the genus Candida the objective of
this study was to compare the hemolytic activity between C. albicans and other Candida species. A total of (100) Candida
isolates representing (4) species are examine for their respective responses to an in hemolytic test. All strains of Candida
specie isolated from the oral cavity of patients in Jumhuri Hospital in Kirkuk city. The four species isolated were: C. albicans,
C. glabra, C. krusei and C. kefyr. Production of Hemolysin was evaluated on sabouraud dextrose agar containing
chloramphenicol blood and glucose. A loop full of pure Candida culture was spot-inoculated and incubated for 24 hours at
37°C. Hemolytic activity was defined as the formation around the colonies of a translucent halo. 10 strains of C. albicans four
strains of C. glabra 43 strains of C. krusei and 1 strain if C. kefyr subsequently that were studied produced hemolysins. Over
all this study showed that hemolytic activity was detected in all isolates with minor differences seen between them the
highest hemolytic activity detected in C. krusei (20-35mm) followed by C. albicans produces (25-33mm) while C. glabra (19-
31mm) and the lowest hemolytic activity was detected in C. kefyr (14-16mm).
نهان بهاءالدين جعفر | Nehan Bahaaldden Jafar | 12859