عنوان المقالة:Immunomodulation of Polysaccharides Extracted From Wild Lycium barbarum Iraqi plant Immunomodulation of Polysaccharides Extracted From Wild Lycium barbarum Iraqi plant
Zainab Yaseen Mohammed1, , Ahmad Rushdi Abdullah2,
English Authors
Zainab Yaseen Mohammed1, , Ahmad Rushdi Abdullah2,
Abstract
This study demonstrates the favorable effects of Iraqi wild type Lycium barbarum
active component as an immunomodulation agent. The fruit of Iraqi Lycium barbarum is rich
with Polysaccharides, which were investigated qualitatively and quantitatively in the present
study. The extracted polysaccharides total content calculated, as glucose was 3.4mg/g dried
fruits.
The Immunomodulation effect for the extracted polysaccharides on normal human
peripheral blood lymphocytes showed an increasing in lymphocytes proliferation significantly
when it tested by MTT assay. The immune stimulating effect of the polysaccharides extract
caused alteration in both IL-10 and TNF-α levels. After 2 hours of exposure to the extracted
polysaccharides at concentrations (250 and 500 µg/ml), the normal human blood lymphocytes
showed an elevation in IL-10 level against TNF-α level while the apposite results developed
after 4 hours of exposure and both estimations were done by ELISA technique.
Abstract
This study demonstrates the favorable effects of Iraqi wild type Lycium barbarum
active component as an immunomodulation agent. The fruit of Iraqi Lycium barbarum is rich
with Polysaccharides, which were investigated qualitatively and quantitatively in the present
study. The extracted polysaccharides total content calculated, as glucose was 3.4mg/g dried
fruits.
The Immunomodulation effect for the extracted polysaccharides on normal human
peripheral blood lymphocytes showed an increasing in lymphocytes proliferation significantly
when it tested by MTT assay. The immune stimulating effect of the polysaccharides extract
caused alteration in both IL-10 and TNF-α levels. After 2 hours of exposure to the extracted
polysaccharides at concentrations (250 and 500 µg/ml), the normal human blood lymphocytes
showed an elevation in IL-10 level against TNF-α level while the apposite results developed
after 4 hours of exposure and both estimations were done by ELISA technique.
احمد رشدي عبد الله | Dr Ahmed Rushdi Abdullah | 2595