عنوان المقالة:Estimate of JAK 2 V617F Mutation and Activity of Catalase Enzyme in Myeloproliferative Neoplasm Estimate of JAK 2 V617F Mutation and Activity of Catalase Enzyme in Myeloproliferative Neoplasm
Heba Rushdi Abdullah, Perry Habib Saifullah, Ziad Ahmed Shebib and Ahmed Rushdi Abdullah
English Authors
Heba Rushdi Abdullah, Perry Habib Saifullah, Ziad Ahmed Shebib and Ahmed Rushdi Abdullah
Abstract
Background: Myeloproliferative neoplasms (MPNs) are a range of clonal hematological diseases
with overlapping features, a specific mutation in the JAK2 gene, which encodes a tyrosine Kinase
has been shown to be associated with the myeloproliferative phenotypes (PV, ET and PMF). Aim:
Estimate the proportion of JAK2 V617F mutant gene and estimate the significance of antioxidant
enzyme (Catalase) is mainly associated with increased oxidative stress this is resulting increase of
the free oxygen radicals and relate it with suspected myeloproliferative neoplasms (MPNs) in Iraqi
patients. Materials and Methods: Total of (51) patients with suspected myeloproliferative neoplasms
(MPNs) and 20 healthy individuals were analyzed for the JAK2 V617F mutation. After DNA
extraction, detection of the mutation was done using (ARMS) PCR amplification, activity of
Catalase was measured spectrophotometrically. Results: Of 51 patients, the JAK2 V617F mutation
(V617F) was detected in 33 out of 42, with PV (81%), and four of patients with ET and PMF (40%
and 50%, respectively). The prevalence of this mutation is more associated with male than female
about (62%). Catalase activity was found highly significant (P< 0.01) among suspected MPN
patients when compared with control group. Conclusion: JAK2 V617F mutation screening can be
incorporated in the initial estimate of patients suspected of having MPNs. Catalase enzyme used a
biomarker of enzymatic alteration in MPN Iraqi patients.
Abstract
Background: Myeloproliferative neoplasms (MPNs) are a range of clonal hematological diseases
with overlapping features, a specific mutation in the JAK2 gene, which encodes a tyrosine Kinase
has been shown to be associated with the myeloproliferative phenotypes (PV, ET and PMF). Aim:
Estimate the proportion of JAK2 V617F mutant gene and estimate the significance of antioxidant
enzyme (Catalase) is mainly associated with increased oxidative stress this is resulting increase of
the free oxygen radicals and relate it with suspected myeloproliferative neoplasms (MPNs) in Iraqi
patients. Materials and Methods: Total of (51) patients with suspected myeloproliferative neoplasms
(MPNs) and 20 healthy individuals were analyzed for the JAK2 V617F mutation. After DNA
extraction, detection of the mutation was done using (ARMS) PCR amplification, activity of
Catalase was measured spectrophotometrically. Results: Of 51 patients, the JAK2 V617F mutation
(V617F) was detected in 33 out of 42, with PV (81%), and four of patients with ET and PMF (40%
and 50%, respectively). The prevalence of this mutation is more associated with male than female
about (62%). Catalase activity was found highly significant (P< 0.01) among suspected MPN
patients when compared with control group. Conclusion: JAK2 V617F mutation screening can be
incorporated in the initial estimate of patients suspected of having MPNs. Catalase enzyme used a
biomarker of enzymatic alteration in MPN Iraqi patients.
احمد رشدي عبد الله | Dr Ahmed Rushdi Abdullah | 2771