Amir Fadhil Al-Tu'ma, Zuhair Mohammed Ali Jeddoa, Hedef Dhafir El-Yassin, Dhafer A.F. Al-Koofee
Abstract
Background: Type 1 diabetes mellitus (T1DM) is an autoimmune disease, arising through a complex interaction of both genetic and immunologic factors. There is an association between human leukocyte antigen (HLA) complexandT1DMinvariouspopulations.ThealleleofHLA-DQA1genepolymorphismassociatedwithT1DMis HLA-DQA1*0501. The blood glycated hemoglobin (HbA1c) level provides the average blood glucose levels during two to three months, which is the predicted half-life of red blood cells (RBCs). Objective: To investigate the molecular basis of the association of HLA- DQA1*0501 allele with HbA1c levels in children with T1DM, in the Kerbala province of Iraq. Materials andmethods: Thisisacase-control studyconducted on125T1DMpatients, including66males(52.8%) and 59 females (47.2%), with the mean age of 10.92 ± 3.78years and 100 healthy controls, including 57 males (57%) and 43 females (43%), with the mean age of 8.4 ± 3.53years, randomly recruited from the Kerbala province of Iraq. Genotyping of HLA was performed on genomic DNA, using polymerase chain reaction sequence-specific primers (PCR-SSP). Results: The frequency of HLA-DQA1*0501 allele in T1DM patients was 71.2%, and in apparently healthy control was 29%. The association between HbA1c level and HLA-DQA1*0501 allele was significant (P value ≤.01). Conclusion: The HLA-DQA1*0501 allele was highly associated with the risk of T1DM pathogenesis. A high significant correlation between the blood HbA1c level and HLA- DQA1*0501 allele was found.
ظافر عبد المهدي فيصل الكوفي | Dhafer A.F. Al-Koofee | 5090