عنوان المقالة: An Optimization and Common Troubleshooting Solving in Polymerase Chain Reaction Technique
ظافر عبد المهدي فيصل الكوفي | Dhafer A.F. Al-Koofee | 5089
Publication Type
Journal
Arabic Authors
English Authors
Shaden M. H. Mubarak; Dhafer A. F. Al-Koofee; Ohood A Radhi; Jawad Mohammed Ismael; Zubaida Falih Al-Zubaidi
Abstract
Many genetic researches now relies on the study of variants in genetic material through different, diverse of universal Polymerase Chain Reaction (PCR) methods. In that context, we are putting a highlighted on the most important fundamental aspects of PCR technology, which help researchers to clarify and reduce the majority problems and difficulties may face them in the laboratory work of any kind of PCR technology in general.
Publication Date
2/29/2020
Publisher
Sys Rev Pharm
Volume No
10
Issue No
2
File Link
تحميل (103 مرات التحميل)
Keywords
PCR optimization; primer design; melting temperature; PCR troubleshooting; GC-high; DMSO; genetics.
رجوع