عنوان المقالة:استخدام العزلات البكتيرية فى ازالة النيكل كعنصر ثقيل Evaluation of nickel tolerance by identified Pseudomonas aeruginosa isolated from Egyptian polluted soils
Mamdouh S. Elgamal, Ayman Farrag Ahmed and Salah Abdelbary
Abstract
استخدام العزلات البكتيرية فى ازالة النيكل كعنصر ثقيل
Abstract
In Egypt, heavy metal pollution causes environmental problems to human, animal, plant and ecosystem. This study aims to solve this problem by bacterial isolates. The bacterial isolates were isolated from 12 polluted site soil mainly industrial soils. The total content of Nickel was measured in soil and recorded the dangerous values to environment above safe international ratios. Also, total count of bacterial colonies recorded significant distribution in all soils. Isolation and screening of nickel tolerant bacterial isolates were recorded that some bacterial isolates were tolerate high nickel concentration up to 600 ppm. MTC, MIC and Tolerance index indicated that there was one bacterial isolate were tolerated high concentration of nickel. This bacterial isolate identified as Pseudomonas aeruginosa after morphological, biochemical and molecular identification. Also, it included that study the effect of nickel on Pseudomonas aeruginosa by using TEM. In finally, it obtained detection of plasmid as indicator for nickel tolerance by Pseudomonas aeruginosa.
د. صلاح عبدالباري عبدالحميد | Dr. Salah Abdelbary Abdelhameed | 3233