Online International Interdisciplinary Research Journal
Abstract
To investigate the comparative roles of anti-inhibin and eCG-hCG treatment on
uterine implantation sites and litter size in virgin female rats, 120 virgin cycling
female rats, aged 56 days and weighted 150-170 g., were randomly assigned to three
equal groups. At late metaestrus and 54h later, the females were supplemented with
intraperitoneal injection of 1>g anti-inhibin and 100>l normal saline, respectively (AI
group), 20IU eCG and 10IU hCG, respectively (eCG group), and 100>l twice,
respectively (Control). After mating, blood samples were obtained daily from 6
females of each group, at the first 4 days, for hormonal assay (FSH, LH, 17Bestradiol,
and prolactin). At day 5 of pregnancy, 6 females from each group, were
injected with 1% (w/v) pontamine blue (500 >l, iv). After 15 min., the females were
sacrificed for counting of uterine implantations. The remaining 18 females continued
to the end of gestation. Litter numbers were counted. Serum FSH in AI females
registered higher levels than others throughout the four days of pregnancy, whereas
LH levels showed no differences, except day one which recorded significant elevation
in eCG group. Prolactin levels increased significantly in AI group throughout the four
days of pregnancy. Estradiol concentrations showed insignificant differences among
groups except day one which showed significant elevation in AI group. Uterine
implantation sites increased significantly in AI group compared with others, also,
eCG group recorded higher number than control. In conclusion treatment of virgin
female rats with anti-inhibin, at late metastrus, increases the reproductive efficiency
and multiple births.
Publication Date
1/27/2016
Publisher
Online International Interdisciplinary Research Journal
حاتم عبدالكريم جبر الشويلي | Hatim Alshwilly | 4883