عنوان المقالة: Synthesis, structure, in vitro anti-cancer, DNA binding and cleavage activity of palladium(II) complexes based on isatin thiosemicarbazones derivatives
Amna Qasem Ali, Siang Guan Teoh, Naser Eltaher Eltayeb, Mohamed B. Khadeer Ahamed, A.M.S. Abdul Majid, Arabya Abdelsalam A
Abstract
Six novel palladium(II) complexes of a thiosemicarbazone Schiff base with isatin
moiety (PdL1 to PdL6) were synthesized by the reaction of palladium(II) with the
following: (Z)‐2‐(2‐oxoindolin‐3‐ylidene)‐N‐phenylhydrazinecarbothioamide (L1H),
(Z)‐2‐(5‐methyl‐2‐oxoindolin‐3‐ylidene)‐N‐phenylhydrazinecarbothioamide (L2H),
(Z)‐2‐(5‐fluoro‐2‐oxoindolin‐3‐ylidene)‐N‐phenylhydrazinecarbothioamide (L3H),
(Z)‐N‐methyl‐2‐(5‐nitro‐2‐oxoindolin‐3‐ylidene)hydrazinecarbothioamide (L4H),
(Z)‐N‐methyl‐2‐(5‐methyl‐2‐oxoindolin‐3‐ylidene)hydrazinecarbothioamide (L5H)
and (Z)‐N‐ethyl‐2‐(5‐methyl‐2‐oxoindolin‐3‐ylidene)hydrazinecarbothioamide (L6H).
The structures of these complexes were characterized using elemental analysis and
infrared, UV–visible, 1H NMR and mass spectroscopies. The structure of PdL5 was
further characterized using single‐crystal X‐ray diffraction. The interaction of these
complexes with calf thymus DNA was characterized with a high intrinsic binding
constant (Kb = 5.78 × 104 to 1.79 × 106M−1), which reflected the intercalative activity
of these complexes towards calf thymus DNA. This result was also confirmed from
viscosity data. Electrophoresis studies revealed that complexes PdL1 to PdL6 could
cleave DNA via an oxidative pathway in the presence of an external agent. Data
obtained from an in vitro anti‐proliferative study clearly established the anticancer
potency of these compounds against the human colorectal carcinoma cell line HCT
116.
د. عربية عبد السلام عبد النبي المطلب | Dr. Arabya abdelsalam Abdunabi Almutaleb | 8218