عنوان المقالة: Molecular detection and phylogenetic analysis of Vibrio cholerae genotypes in Hillah, Iraq

أ.د. أمل طالب عطيه السعدي | Prof. Dr. Amal Talib Atiyah Al Sa'ady | 6499

---

Publication Type

Journal

Arabic Authors

أ.د. امل طالب السعدي + د.خمائل عدنان + د.زهراء قيس

English Authors

4 Amal Talib Al Sa’ady + K. A. Baqer + Z. K. S. Al-Salim

Abstract

Vibrio cholerae is a cause of serious endemic diarrhoea associated with cholera in many regions in the world. A total of 256 stool and rectal swabs were collected from patients suspected to have cholera admitted to three hospitals in Hillah, Babylon Governorate, Iraq, for the period 1 September to 29 December 2017. After the routine culture of samples for isolation and identification of V. cholerae isolates, PCR was performed for molecular detection of V. cholerae isolates based on 16S ribosomal RNA gene. Toxigenicity was detected by RTX toxin genes. PCR technique emphasized molecular detection of V. cholerae for eight isolates. Only two isolates (25%) possessed both the rtxA and rtxC genes, while only three isolates (37.5%) possessed the rtxB gene. DNA sequencing was performed for the eight isolates via analysis and phylogenetic tree. The observed bacterial variants were compared to their neighbour homologous reference sequences using the National Center for Biotechnology Information (NCBI) BLAST server (Basic Local Alignment Search Tool; https://blast.ncbi.nlm.nih. gov/Blast.cgi). The findings indicated that the eight investigated isolates of V. cholerae were positioned in three different phylogenetic positions. Partial sequence dissimilarities were reported between GenBank isolate accession number MK212155.1 and these six clustered GenBank accession numbers of the same species. For the first time in Babylon Governorate, Iraq, the molecular assay, sequencing and phylogenetic tree are reported for V. cholerae and their toxins isolated during the 2017 cholera outbreak. © 2020 The Authors. Published by Elsevier Ltd.

Publication Date

8/7/2020

Volume No

Issue No

DOI

https://doi.org/10.1016/j.nmni.2020.100739

File Link

تحميل (61 مرات التحميل)

Keywords

Al Hilla (Iraq), molecular detection, outbreak 2017, phylogenetic tree, sequencing, Vibrio cholerae