عنوان المقالة:The role of Van A gene in the resistance in the resistance of nuc-gene positive vancomycin resistant Staphylococcus aureus isolated from patients with skin infection The role of Van A gene in the resistance in the resistance of nuc-gene positive vancomycin resistant Staphylococcus aureus isolated from patients with skin infection
Mushtak T.S. Al-Ouqaili (1) , Shaymaa H.M. Al-Kubaisy(2) & Narjis F.I. H. Al-Ani(3)
Abstract
Vancomycin resistance Staphylococcus aureus (VRSA) have become an increasing problem
worldwide. This study has been undertaken for detection the role of VanA gene in vancomycin resistant
S. aureus isolated from patients with skin infections.Seventy five specimensobtained from patients
admitted to Dermatology Department in Al-Ramadi Teaching Hospitaland outpatient from Private
clinics were studied. The suspected Staphylococcal colonies were bacteriologically identified and
confirmed by biochemical test. Preliminary agar screening plate for detection of vancomycin resistant
S. aureus which includes Muller Hinton agar containing 6mg/100ml vancomycin was performed. Also,
broth dilution technique to detect minimal inhibitory concentration for VRSA was achieved. Further,
Amplification of nuc and vanA genes by polymerase chain reaction was also performed.Out of Fourty
fiveisolates of Staphylococci, 38 (84.4%) were diagnosed bacteriologically as S. aureus. Of these
isolates, 27 (71.1%) isolates were resistant to oxacillin. Vancomycin resistant S. aureus were detected
in 10 (37%) of them while the remaining, 1728.9%) were vancomycin sensitive. Among 10 (37%) of
Vancomycin resistant S. aureus, 6 (60%) of them showed positive result for the presence of VanA
gene.The increase in vancomycin resistance among MRSA and excessive use of antimicrobial agents
have worsened the sensitivity. Also, the study suggested that nuc gene is cut off molecular diagnostic
tool for confirmed diagnosis of S. aureus. Further, VanA gene play an important role in nuc gene
positive vancomycin resistant S. aureus
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 6578