عنوان المقالة:Molecular Analysis for Azoospermia Factor Microdeletions in the Y chromosome for Azoospermic and Severe Oligospermic Infertile Iraqi Patients Molecular Analysis for Azoospermia Factor Microdeletions in the Y chromosome for Azoospermic and Severe Oligospermic Infertile Iraqi Patients
Mohammed. N Al-Qaisi, Mushtak. T Al-Ouqaili, Duraid. T Al-Hadithi
Abstract
Defined infertility as incapability of a married couple tᴏ have children for the
one-year ᴏf unprotected intercourse. Y chromosomal microdeletions are the
second greatest common genetic reason of men sterility. This research aims to
find the prevalence of AZF Y chromosome microdeletions in azoospermic and
severe oligospermia patients. Further, to evaluate the prevalence types of
AZF/c sub-region microdeletions in patients with AZF/c deletion in Iraq. A
total of 75 infertile Iraqi males and 25 control were included in this study. The
DNA samples were extracted, and they were analysed for AZF microdeletions
by utilizing eight sequence-tagged sites through a q/real-time PCR system.
After that, partial AZF/c deletion sub-region was investigated using four
specific primers. These markers were chosen according to the EAA/ EMGQ
recommendations. Out of 75 infertile patients, 46 patients (61.33%) revealed
AZF microdeletions in the Y chromosomal with at least one STS deletion for
one or more AZF regions. (32.6%) of patients with microdeletions observed in
three regions AZFabc. Out of 24 patients who have AZF/c microdeletions
(37.5%) were exhibited b2/b4 deletion (complete AZF/c deletions), (58.3%)
were showed gr/gr microdeletion (partial AZF/c deletions. and (4.1%) with
b2/b4 deletion continues the terminal heterochromatin region. The incidence
of classical AZF microdeletions in our study subjects is high. In our study
population, gr/gr partial AZF/c microdeletions were higher than b2/b4
complete AZF/c deletion. The mean levels of sex hormones in azoospermic
sterile patients with AZF microdeletion were higher than the mean of
azoospermic sterile men without deletion of AZF.
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 8272