عنوان المقالة:Effect of pyocyanin as secondary metabolite on pseudomonal biofilm and in increasing the resistance degree to antipseudomonal agents Effect of pyocyanin as secondary metabolite on pseudomonal biofilm and in increasing the resistance degree to antipseudomonal agents
Al-Moghira Khairi Al-Qaysi1 , Mushtak T. S. Al-Ouqaili2 *, Safaa Abed Latef Al-Meani3
Abstract
Background: Pseudomonas aeruginosa produced the maximum amount of pyocyanin pigment as a secondary metabolite as
a virulence factor that responsible for increase pathogenesis. The study aims to improve our understanding of the emergence
of pseudomonal biofilm formation by pyocyanin production. The correlation between minimal inhibitory concentration
(MICs) values of ceftazidime, piperacillin, and ciprofloxacin with pyocyanin production is also targeting in this study.
Materials and Methods: A total of 96 isolates from patients with catheter-associated urinary tract and wound infections
were included in this study. Pyocyanin production was determined. The antimicrobial susceptibility tests including, Kirby–
Bauer and E-test, were estimated. Finally, the detection of biofilm production, which includes microtiter plate assay and
biofilm formation on FBCL, was done. Results: One hundred thirty-eight isolates of P. aeruginosa, isolates that produced
biofilm were 96 (69.5%). There is a progressive relationship between pyocyanin production level and biofilm production.
Furthermore, there is a relationship between MICs values of ceftazidime and pyocyanin production level. Conclusion: Our
suggestion showed that pyocyanin could be one of the factors that help to induce biofilm formation. Wherefore, sessile cells
were showed an increase in isolates that have higher pyocyanin levels more than others. Furthermore, pyocyanin increases
the resistance against ceftazidime, which could be of clinical significance.
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 7051