عنوان المقالة:Study of the Cytotoxic Effects of Aflatoxin on Hematopoietic Stem Cells Study of the Cytotoxic Effects of Aflatoxin on Hematopoietic Stem Cells
Musleh Mohammed Hamada1*, Al-Kubaisi Salah M.A.2 and Al-Ouqaili Mushtak T.S.3
Abstract
Aflatoxins (AFs) are secondary metabolites having a
high cytotoxic potential and have an active
carcinogenic compound. This study aimed to isolate
hematopoietic stem cells (Precursors of mononuclear
stem cells) from human umbilical cord blood.
Collection of umbilical cord blood samples from cord
blood was accomplished immediately after delivery,
while the placenta is intrauterine by used closed system
method. The mononuclear cells (MNCs) were obtained
from umbilical cord blood by using Buffy coat method.
The previous cells have been Hematopoietic Stem Cells
(HSCs) used in the cytotoxicity test of AF extract. The
mononuclear cells were cultured in DMEM medium
with 10% FCS. An experimental study was conducted
in vitro on these cells to confirm the toxic effects of AF
with different concentrations of AFs.
The results showed that the inhibition rate increases
seriously with the increase of AF concentration.
Aspergillus flavus in peanut produces the highest
concentration of AF, while in soil, contamination with
crude oil A. flavus produces the least concentration of
AF. Additionally, the concentration of 1 μg /ml of AF
extracted from the aflatoxigenic isolates which can kill
100% of hematopoietic stem cells.
الأستاذ الدكتور مشتاق طالب صالح الندا | Dr. Mushtak T. S. Al-Neda | 7050