Marwa F.M.S. Atef, Abdul Rahman A. Bin Yahya, Adel A.M. Saeed
Abstract
Kidney stone is one of the most prevalent
diseases worldwide. Phytochemicals are responsible for
the medicinal activity of plant species. They have the
ability for curing various ailment and possesses potential
anti-inflammatory, anti-bacterial, anti-oxidant, and antifungal properties. Natural products from medicinal
plants, either as pure compounds or as extracted out,
provide opportunities for new drug leads because of the
unmatched availability of chemical diversity. Due to the
rising demand for chemical diversity nowadays in
screening programs, seeking therapeutic drugs from
herbal products, are quite interesting throughout the
world. The present study aims at studying the antiurolithiatic activity of aqueous, methanolic, and hot
aqueous extracts of the leaves of Indigofera oblangifolia
(Fabaceae),
leaves and barks of
Capparis
Catrilaginea )Capparisaceae( and vegetable collection of
Fagonia indica Burm.f. (Zygophyllaceae). First, in-vitro
study was conducted to assess anti-urolithiatic effect of
plants for all extracts with a standard drug namely
Cystone as a control. Turbidity method and calcium
oxalate dissolution method were practiced to access the
inhibition of stone formation and dissolution of stone
crystals respectively. Secondly, extracts were prepared
and arranged in different concentrations. The
homogenous precipitation method was used to prepare an
artificial stone such as calcium oxalate and a semipermeable membrane of eggs was used as dissolution
bags. Dissolution models were incubated for 72hrs and
after that, the entire content in dissolution bags was
estimated spectrophotometrically. The inhibitory activity
of extracts on the nucleation of calcium oxalate crystals
and the rate of aggregation in calcium oxalate crystals
were determined by spectrophotometric assay besides a
titration method. Aqueous & alcoholic extracts of
Indigofera oblangifolia leaves showed the highest
solubility, inhibition, and non-formation of calcium
oxalate followed by Capparis Catrilaginea bark, the
results were very close to the Cystone control. On the
other hand, the lowest effects were for Fagonia indica
Burm.f. leaves and Capparis Catrilaginea leaves.
Indigofera oblangifolia leaves and Capparis Catrilaginea
bark in some extracts exhibited significant in-vitro antiurolithiatic activity. Aqueous and alcoholic extracts of Indigofera oblangifolia leaves and Capparis Catrilaginea
bark had similar effects as commonly used Cystone
medicine for preventing as well as treating renal stones,
even in its crude form. Results guide us for the further
detailed investigation and development of new drugs from
these medicinal plants.
Publication Date
3/30/2023
Publisher
International Journal of Innovative Science and Research Technology
د. عادل أحمد محمد سعيد | Dr. Adel Ahmed Mohammed Seaad | 13933